TABLE 2

Relative abundance of highly enriched OTUs detected in heavy-density fractions of SIP experiments of PP and BHS soils incubated with B. indica [13C]EPSd

Soil sample, time of collection, and OTURelative % abundance in:Fold enrichment (% abundance in heavy DNA/original soil)Taxonomy (phylum, class)Closest relative by BLAST analysis% identity to the closest relative
Heavy DNA fractionOriginal soil
PP soil, 80 days
    1577a62.00.0541,148Planctomycetes, PhycisphaeraeSchlesneria paludicola85
    122927.08UD>488Candidate division OD1<80
    96122.780.011254MLE1-12Microcystis ichthyoblabe (Cyanobacteria)87
    38481.40UD>96ArmatimonadetesFrankia alni (Actinobacteria)87
    75411.080.04325Planctomycetes, PlanctomycetaciaSingulisphaera acidiphila95
PP soil, 111 days
    122015.740.011434Planctomycetes, PlanctomycetaciaAquisphaera giovannonii93
    11666b3.52UD>320Planctomycetes, PlanctomycetaciaAquisphaera giovannonii91
BHS soil, 80 days
    26137.180.010658Planctomycetes, PhycisphaeraeSchlesneria paludicola84
    53412.57UD>236Verrucomicrobia, SpartobacteriaRoseimicrobium gellanilyticum88
    4249c2.46UD>223Planctomycetes, PhycisphaeraeSchlesneria paludicola84
  • a Also includes the closely related OTUs 11375, 12969, 505, 4724, 10597, and 3328 (see Table S2 in the supplemental material).

  • b Also includes the closely related OTUs 1598, 3075, and 778 (see Table S2 in the supplemental material).

  • c Also includes the closely related OTU 10087 (see Table S2 in the supplemental material).

  • d Only OTUs with >1% relative abundance in the heavy fraction (generally >100 reads) and enriched at least 25-fold compared to the amount in unincubated soil are shown. On the basis of a Z test of ratios, all significance values (chance of sampling these differences from the same population) are <0.001. UD, no reads were detected in the original soil; the minimum fold enrichment in these cases was calculated by assuming 1 read in the original soil.