TABLE 2

Thermodynamic parameters of ligand binding derived from the microcalorimetric titration of the McpP LBD with different ligandsa

LigandKA (M−1)KD (μM)ΔH (kcal/mol)nb
Acetate(2.9 ± 0.4) × 10434 ± 5−5.11 ± 3.10.77 ± 0.4
Propionate(2.9 ± 0.3) × 10434 ± 4−5.35 ± 3.31.37 ± 0.6
Pyruvate(2.6 ± 0.2) × 10439 ± 3−9.94 ± 2.60.89 ± 0.2
l-Lactate(9.3 ± 1) × 103107 ± 11−5.20 ± 4.11.26 ± 0.8
  • a Data are means and standard deviations from three experiments.

  • b Stoichiometry of binding of ligands to the McpP LBD.