TABLE 1

Strains and plasmids used in this studya

Bacterial strain or plasmidDescriptionSource or reference
Bacterial strains
    E. coli DH5αF ϕ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK, mK+) phoA supE44 λ thi-1 gyrA96 relA1Invitrogen, Carlsbad, CA
    E. coli DH11SmcrA Δ(mrr-hsdRMS-mcrBC) Δ(lac-proAB) Δ(rec1398) deoR rpsL srl- thi-/FproAB+ lacIqZΔM15Invitrogen, Carlsbad, CA
    L. casei BL23Previously known as L. zeae ATCC 393 pLZ1514
    L. rhamnosus GG (CMC)Wild typeCMC
    L. rhamnosus GG (ATCC 53103)Wild typeATCC
    L. rhamnosus GG (NCC 3003)Wild typeNCC
    L. rhamnosus GG (Gefilus)Wild typeGefilus (Valio Ltd.)
    L. rhamnosus GG (UT)Natural welFE mutantUT
    L. rhamnosus GG (EM233)GG (UT) with the original ARP1 cassette (anchor domain 243-aa PrtP of L. casei) integrated into the chromosomeThis study
Plasmids
    pIAV7Broad-range vector, Err, lacZ, pWV01 replication origin24
    pEM233pUC19E-borne six1, A2-int-attP six2, with expression cassette of pAF900-ARP117
    pEM94pG+host9-borne β-recombinase gene, carrying Cmr47
    pSP72Cloning vectorPromega
    pAF900-ARP1Original ARP1 cassette (anchor domain 243-aa PrtP of L. casei) in pIAV717
    pLB11Modified ARP1 cassette (anchor domain 450-aa PrtP GG) in pIAV7This study
    pLB12Modified ARP1 cassette (anchor domain 500-aa PrtP GG) in pIAV7This study
    pLB13Modified ARP1 cassette (anchor domain 550-aa PrtP GG) in pIAV7This study
    pLB19Modified ARP1 cassette (anchor domain 865-aa PrtP GG) in pIAV7This study
    pLB20Modified ARP1 cassette (anchor domain 1,275-aa PrtP GG) in pIAV7This study
    pLB31welFE genes in pIAV7This study
  • a CMC, Christian Medical College, Vellore, India; ATCC, American Type Culture Collection; NCC, Nestlé Culture Collection; UT, University of Tartu, Estonia.