TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype, phenotype, or characteristic(s)Source
X. nematophila strains
    AN6/1Wild-type, phase 1 variant; AmprLaboratory stock
    ΔxcnKL strainAN6/1 ΔxcnKL::KmrD. Park
    FAN6/1 XNC1_2467::CmrThis study
    ngrA strainAN6/1 XNC1_1028::CmrThis study
    ΔxcnKL:A strainAN6/1 ΔxcnKL::Kmr XNC1_2040::CmrThis study
    ΔxcnKL:B strainAN6/1 ΔxcnKL::Kmr XNC1_2783::CmrThis study
    ΔxcnKL:C strainAN6/1 ΔxcnKL::Kmr XNC1_1763::CmrThis study
    ΔxcnKL:D strainAN6/1 ΔxcnKL::Kmr XNC1_2228::CmrThis study
    ΔxcnKL:E strainAN6/1 ΔxcnKL::Kmr XNC1_2300::CmrThis study
    ΔxcnKL:F strainAN6/1 ΔxcnKL::Kmr XNC1_2467::CmrThis study
E. coli S17-λpirrecA thi pro hsd(R M+); RP4-2Tc::Mu Km::Tn7 in the chromosomeLaboratory stock
E. faecalisManduca sexta gut isolate
S. saprophyticusManduca sexta gut isolate
Plasmids
    pSTBlue-1Cloning vector; Ampr KmrNovagen
    pKnock-CmrBroad-host-range suicide vector; Cmr RP4 oriT oriR6KD. Saffarini
    pKnock-AInternal fragment of XNC1_2040 cloned into pKnock-CmrThis study
    pKnock-BInternal fragment of XNC1_2783 cloned into pKnock-CmrThis study
    pKnock-CInternal fragment of XNC1_1763 cloned into pKnock-CmrThis study
    pKnock-DInternal fragment of XNC1_2228 cloned into pKnock-CmrThis study
    pKnock-EInternal fragment of XNC1_2300 cloned into pKnock-CmrThis study
    pKnock-FInternal fragment of XNC1_2467 cloned into pKnock-CmrThis study