TABLE 2

Primers used in this study

Gene, cluster, or vectorPrimerSequence (5′–3′)Use
ngrAXNC1_1028 FCATGTCATGCTGGCCTATTTTGMutant construction
XNC1_1028 RCAAATAGTGTCAGGCCAGATTGGMutant construction
XNC1_1028 OFACCTCTACTCAAGTCACTACMutant screening
Cluster AXNC1_2040 FTCTCTCAGTACGACTCAGCAAGMutant construction
XNC1_2040 RCTATCTTGTCTGTAGCGTTGTGAGMutant construction
XNC1_2040 OFTCATGCAATCGTATCAACAACGMutant screening
Cluster BXNC1_2783 FGCCAAACGATTAGAAGAAGCTCTCMutant construction
XNC1_2783 RACAGTGAATTGTGCAGGATCTTGMutant construction
XNC1_2783 OFTCAAATTGACCCTGACAAACGMutant screening
Cluster CXNC1_1763 FCACTTACGGATATGCAGCAGGMutant construction
XNC1_1763 RGTGTTGTTGCTGCCAATTGACMutant construction
XNC1_1763 OFTTTGGGAAAACTATTCTGATGTTGMutant screening
Cluster DXNC1_2228 FACAGCATACCGCCAGAATTGMutant construction
XNC1_2228 RTGAACCTTTAGCACTGGCAATMutant construction
XNC1_2228 OFTCAATGAGGCGTTAAATCAGGGMutant screening
Cluster EXNC1_2300 FCATCATGACAATATACCACCGGAACMutant construction
XNC1_2300 RAAGTTCATCCCAATGCTCTGTGMutant construction
XNC1_2300 OFCTTTAAGCCAAGGAGTTACCCTGMutant screening
Cluster FXNC1_2467 FGACAGGCTCTCAATGGCACMutant construction
XNC1_2467 RGGACTCTGTCACTTTGGTTGATACMutant construction
XNC1_2467 OFTGCCATAAAAGAGACGCTCCAGMutant screening
pKnockpKnock-FACACAGGAACACTTAACGGCTGACMutant screening
pKnock-RTGCGAAGTGATCTTCCGTCAGAGMutant screening
pSTBlue-1SP6ATTTAGGTGACACTATAGMutant screening
T7 promoterCTAATACGACTCACTATAGGGMutant screening