TABLE 3

Sampling sites, numbers (ranges) of fecal indicator bacteria, and real-time PCR positive/negative results for host-associated molecular markers

Sampling siteaNo. of FIB (CFU/100 ml)Detection of molecular markersb
E. coliEnterococcus spp.CowM3HF183GFDHorse
BR112–183–6− − −− − −+ + +− − −
BR251–8019–25+ + +− − −− − −− − −
BR3242–340510–600− − −+ + ++ + +− − −
BR4395–480522–580− − −+ + ++ + +− − −
BR5121–160174–208− − −− − −+ + ++ + −
BR670–103150–182− − −− − −+ + +− − −
  • a Triplicate samples from each site were tested.

  • b −, negative for the marker; +, positive for the marker. The result for each of the triplicate samples tested is shown.