TABLE 2

Kinetic parameters of the L. reuteri 121 GTFB-ΔN enzyme with the amylose V substratea

ActivityKm (g · liter−1)kcatb (s−1)kcat/Km (g−1 · s−1 · liter)
Hydrolytic0.50 (±0.04)9.06 ± 0.1818.5 (±1.8)
Transferase0.69 (±0.07)36.22 ± 1.1453.0 (±7.2)
Total0.64 (±0.02)45.04 ± 1.1470.6 (±4.0)
  • a A fixed amount of GTFB-ΔN enzyme (60 nM) was used with various concentrations of amylose V (0.025 to 0.4%, wt/vol) at pH 5.0 and 40°C. The values of the kinetic parameters presented are means ± standard deviations.

  • b A molecular mass of 99 kDa (GTFB-ΔN) was used in the calculation of kcat.