TABLE 3

Amylose V (0.25%, wt/vol) degradation activity (total activity, measured using iodine staining assay) and transglycosylation factors of the L. reuteri 121 GTFB-ΔN enzyme in the presence of different acceptor substratesa

Acceptor substrate (10 mM)Amylose degradation (U/mg)Transglycosylation factorb
None2.81.0
Glucose6.42.2
Maltose7.32.6
Maltotriose7.52.7
Isomaltose13.54.7
Isomaltotriose19.06.7
  • a Experiments were carried out in duplicate, and average data are shown.

  • b The transglycosylation factor is defined as the ratio of amylose degradation rates in the presence/absence of an acceptor substrate.