TABLE 1

Bacterial strains and plasmids

Strain or plasmidRelevant propertiesSource or reference
E. coli DH5αF araD139 Δ(ara-leu)7696 Δ(lac)X74 galU galK hsdR2 mcrA mcrB1 rpsLInvitrogen, CA
S. pneumoniae
    D39Encapsulated strain25
    KB1-69D39 Tet bgaA::PZn-mOrange2This study
    KB1-70D39 Tet bgaA::PZn-tagRFPThis study
    KB1-71D39 Tet bgaA::PZn-mCherryThis study
    KB1-72D39 Tet bgaA::PZn-mKate2This study
    MK119D39 Cam hlpA hlpA-mKate218
    KB1-64D39 Cam hlpA-mCherryThis study
    MK218D39 Cam hlpA hlpA-mCherryThis study
    KB1-65D39 Cam hlpA hlpA-tagRFPThis study
    KB04-03D39 Tet bgaA::PssbB-mOrange2This study
    KB04-04D39 Tet bgaA::PssbB-tagRFPThis study
    KB04-05D39 Tet bgaA::PssbB-mCherryThis study
    KB04-06D39 Tet bgaA::PssbB-mKate2This study
    DRR27D39 Ery ftsZ-mCherryThis study
    DRR28D39 Ery ftsZ-mKate2This study
    DRR54D39 Ery ftsZ-tagRFPThis study
    MK125D39 Cam hlpA hlpA-mKate2 Tet bgaA::PZn-gfp-stkPThis study
    MK289D39 Cam hlpA hlpA-mCherry Tet bgaA::PZn-gfp-stkPThis study
    MK290D39 Cam hlpA, hlpA-tagRFP Tet bgaA::PZn-gfp-stkPThis study
    MK292D39 Ery ftsZ-mCherry Tet bgaA::PZn-gfp-stkPThis study
    MK293D39 Ery ftsZ-mKate2 Tet bgaA::PZn-gfp-stkPThis study
    MK294D39 Ery ftsZ-tagRFP Tet bgaA::PZn-gfp-stkPThis study
Plasmids
    pKB01_sfgfp(Sp)bla tet bgaA::PZn-sfgfp(Sp)6
    pKB01_mOrange2bla tet bgaA::PZn-mOrange2(Sp)This study
    pKB01_tagRFPbla tet bgaA::PZn-tagRFP(Sp)This study
    pKB01_mCherrybla tet bgaA::PZn-mCherry(Bs)This study
    pKB01_mKate2bla tet bgaA::PZn-mKate2(Sp)This study
    pKB01-PssbB-mOrange2bla tet bgaA PssbB-mOrange2(Sp)This study
    pKB01-PssbB-tagRFPbla tet bgaA PssbB-tagRFP(Sp)This study
    pKB01-PssbB-mCherrybla tet bgaA PssbB-mCherry(Sp)This study
    pKB01-PssbB-mKate2bla tet bgaA PssbB-mKate2(Sp)This study
    pJWV25-stkPbla tet bgaA PZn-gfp-stkP19
    pLA18bla tet bgaA::PssbB-luc-gfp26