TABLE 1

Strains and plasmids used in this work

Strain or plasmidDescriptionaReference or source
Strains
    JG274S. oneidensis MR-1, wild type3
    JG2989JG274 ΔfeoEThis work
    JG168JG274 with empty pBBR1MCS-2, Kmr27
    JG2993JG2989 with empty pBBR1MCS-2, KmrThis work
    JG2780JG274 with pBBR1MCS-2::feoE, KmrThis work
    JG2994JG2989 with pBBR1MCS-2::feoE, KmrThis work
    JG2997JG2989 with pBBR1MCS-2::fieF, KmrThis work
    MG1655E. coli K-12, wild typeArkady Khodursky, University of Minnesota
    JG3304MG1655 ΔfieFThis work
    JG3306JG3304 with empty pBBR1MCS-2, KmrThis work
    JG3307JG3304 with pBBR1MCS-2::fieF, KmrThis work
    JG3308JG3304 with pBBR1MCS-2::feoE, KmrThis work
    UQ950E. coli DH5α λ(pir) cloning host; F Δ(argF-lac)169 ϕ80dlacZ58ΔM15 glnV44(AS) rfbD1 gyrA96 (Nalr) recA1 endA1 spoT1 thi-1 hsdR17 deoR λpir+26
    WM3064E. coli conjugation strain; thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir(wt)]26
Plasmids
    pSMV3Deletion vector, Kmr sacB31
    pBBR1MCS-2Broad-host-range cloning vector, Kmr30
    pBBR1MCS-2::feoESO_4475 (feoE), 48 bp upstream, 51 bp downstream, KmrThis work
    pBBR1MCS-2::fieFb3915 (fieF), 76 bp upstream, 26 bp downstream, KmrThis work
  • a AS, amber (UAG) suppressor; wt, wild type.