TABLE 2

Primers used for mutant construction and complementation in this work

PrimerSequenceRestriction site
4475USFGTACGGATCCGCAGAGCGCGTAACACTTCBamHI
4475USRGTACACTAGTCCATTGTATATCAGCTTGGCGSpeI
4475DSFGTACACTAGTGCGACACTGAATCGATTATTCAACSpeI
4475DSRGTACGAGCTCGCTCAGTCACAGCGGCATTAACACSacI
4475CompFGTACGGATCCCGCCAAGCTGATATACAATGGBamHI
4475CompRGTACACTAGTGGCATAACCACTCCTTTGATTGSpeI
ECfieFUSFGATCACTAGTCGATACCATTTTTCTTCGGCSpeI
ECfieFUSRGATCCCGCGGCATAAATACTCCCGCTATCAACSacII
ECfieFDSFGATCCCGCGGCGGTCTATGCTTTCATAATCAGSacII
ECfieFDSRGATCGGATCCCATACGGGAAGCCAGAATACBamHI
ECfiefFGTACGGATCCCAATTTGCCTGCTGCTTAATGCBamHI
ECfiefRGTACACTAGTGCGGGTCTGGCTCTCTTTTATACSpeI