TABLE 2

Strains used in this study

Strain nameStrain IDDescriptionSource or reference
E. coli
    DH5αfhuA2 Δ(argF-lacZ)U169 phoA glnV44 ϕ80lacZΔM15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17Invitrogen
H. pylori
    X47OND79Wild-type strain, also known as X47-2AL, naturally resistant to streptomycin50
    X47 ΔdapD::rpsL-catOND644dapD (HP0626) replaced by rpsL-cat cassette, DAP auxotroph38
    X47 ΔdapDOND653dapD (HP0626) deletion, DAP auxotroph38
    X47 HP0626::FLAGOND372527 bases encoding the FLAG peptide inserted before the HP0626 ORF stop codonThis work
    X47 ΔdapD trpA::rpsL-catOND4515HP0626 deletion, rpsL-cat inserted into HP1277, recipient strainThis work
    X47 ΔdapD trpA::uPtetO1-dapD-FLAGOND4769HP0626 deletion, uPtetO1-dapD-FLAG inserted into HP1277This work
    X47 ΔdapD trpA::uPtetO5-dapD-FLAGOND4770HP0626 deletion, uPtetO5-dapD-FLAG inserted into HP1277This work
    X47 ΔdapD trpA::uPtetO5m1-dapD-FLAGOND4771HP0626 deletion, uPtetO5m1-dapD-FLAG inserted into HP1277This work
    X47 ΔdapD trpA::uPtetO5m2-dapD-FLAGOND4772HP0626 deletion, uPtetO5m2-dapD-FLAG inserted into HP1277This work
    X47 ΔdapD trpA::uPtetO5m3-dapD-FLAGOND4773HP0626 deletion, uPtetO5m3-dapD-FLAG inserted into HP1277This work
    X47 mdaB::rpsL-catOND1052rpsL-cat inserted between HP0630 and HP0631, recipient strain13
    X47 mdaB::ptetR1OND1984ptetR1 inserted between HP0630 and HP0631This work
    X47 mdaB::ptetR2OND1985ptetR2 inserted between HP0630 and HP063113
    X47 mdaB::ptetR3OND1986ptetR3 inserted between HP0630 and HP0631This work
    X47 mdaB::ptetR4OND1987ptetR4 inserted between HP0630 and HP063113
    X47 mdaB::ptetR5OND1988ptetR5 inserted between HP0630 and HP0631This work
    X47 mdaB::ptetR6OND1989ptetR6 inserted between HP0630 and HP063113
    X47 mdaB::ptetR7OND1990ptetR7 inserted between HP0630 and HP0631This work
    X47 mdaB::ptetR4 ΔdapD::rpsL-catOND4457ptetR4 inserted between HP0630 and HP0631, HP0626 replaced by rpsL-cat cassetteThis work
    X47 mdaB::ptetR5 ΔdapD::rpsL-catOND4459ptetR5 inserted between HP0630 and HP6031, HP0626 replaced by rpsL-cat cassetteThis work
    X47 mdaB::ptetR4 ΔdapDOND4467ptetR4 inserted between HP0630 and HP0631, HP0626 deletionThis work
    X47 mdaB::ptetR5 ΔdapDOND4469ptetR5 inserted between HP0630 and HP0631, HP0626 deletionThis work
    X47 mdaB::ptetR4 ΔdapD trpA::rpsL-catOND4501ptetR4 inserted between HP0630 and HP0631, HP0626 deletion, rpsL-cat inserted into HP1277, recipient strainThis work
    X47 mdaB::ptetR5 ΔdapD trpA::rpsL-catOND4503ptetR5 inserted between HP0630 and HP0631, HP0626 deletion, rpsL-cat inserted into HP1277, recipient strainThis work
    X47 mdaB::ptetR4 ΔdapD trpA::uPtetO1-dapD-FLAGOND4774ptetR4 inserted between HP0630 and HP0631, HP0626 deletion, uPtetO1-dapD-FLAG inserted into HP1277This work
    X47 mdaB::ptetR4 ΔdapD trpA::uPtetO5-dapD-FLAGOND4775ptetR4 inserted between HP0630 and HP0631, HP0626 deletion, uPtetO5-dapD-FLAG inserted into HP1277This work
    X47 mdaB::ptetR4 ΔdapD trpA::uPtetO5m1-dapD-FLAGOND4776ptetR4 inserted between HP0630 and HP0631, HP0626 deletion, uPtetO5m1-dapD-FLAG inserted into HP1277This work
    X47 mdaB::ptetR4 ΔdapD trpA::uPtetO5m2-dapD-FLAGOND4777ptetR4 inserted between HP0630 and HP0631, HP0626 deletion, uPtetO5m2-dapD-FLAG inserted into HP1277This work
    X47 mdaB::ptetR4 ΔdapD trpA::uPtetO5m3-dapD-FLAGOND4778ptetR4 inserted between HP0630 and HP0631, HP0626 deletion, uPtetO5m3-dapD-FLAG inserted into HP1277This work
    X47 mdaB::ptetR5 ΔdapD trpA::uPtetO1-dapD-FLAGOND4779ptetR5 inserted between HP0630 and HP0631, HP0626 deletion, uPtetO1-dapD-FLAG inserted into HP1277This work
    X47 mdaB::ptetR5 ΔdapD trpA::uPtetO5-dapD-FLAGOND4780ptetR5 inserted between HP0630 and HP0631, HP0626 deletion, uPtetO5-dapD-FLAG inserted into HP1277This work
    X47 mdaB::ptetR5 ΔdapD trpA::uPtetO5m1-dapD-FLAGOND4781ptetR5 inserted between HP0630 and HP0631, HP0626 deletion, uPtetO5m1-dapD-FLAG inserted into HP1277This work
    X47 mdaB::ptetR5 ΔdapD trpA::uPtetO5m2-dapD-FLAGOND4782ptetR5 inserted between HP0630 and HP0631, HP0626 deletion, uPtetO5m2-dapD-FLAG inserted into HP1277This work
    X47 mdaB::ptetR5 ΔdapD trpA::uPtetO5m3-dapD-FLAGOND4783ptetR5 inserted between HP0630 and HP0631, HP0626 deletion, uPtetO5m3-dapD-FLAG inserted into HP1277This work