TABLE 4

Induction and repression of uPtetO promoters by TetR and revTetRa

PromoterTetRrevTetR
Promoter strength (RUb)Fold increasePromoter strength (RU)Fold decrease
−ATc+ATc−ATc+ATc
uPtetO5m22501,80079901208
uPtetO5m12803,200113,0003808
uPtetO5m38206,60084,9005409
uPtetO53,30010,000310,0003,3003
uPtetO191013,0001412,0002,3005
  • a Quantification of immunoblot signals for Fig. 2A and B was performed to determine the relative strength of each tet promoter. The signal strengths of DapD-FLAG and the upper background bands were determined by densitometry, and the loading-adjusted DapD-FLAG expression levels were calculated by dividing the DapD-FLAG level by the background band level. The results are representative of at least three independent experiments.

  • b RU, relative units.