TABLE 2

In vitro enzyme activities of NADPH-specific alcohol dehydrogenase, acetyl-CoA synthetase, and glucose-6-phosphate dehydrogenase

StrainDescriptionaEnzyme activity (μmol min−1 mg protein)b
NADPH-ADHACSG6PDH
M2390Wild type0.002 ± 0.0000.03 ± 0.000.18 ± 0.08
M6571Gpd Ba-adhE0.003 ± 0.0000.04 ± 0.000.17 ± 0.02
M6951Gpd Ba-adhE Eh-ADH10.031 ± 0.0030.04 ± 0.000.17 ± 0.03
M7888Gpd Ba-adhE Eh-ADH1 pADH1-ZWF1NDc0.03 ± 0.005.46 ± 0.14
M7890Gpd Ba-adhE Eh-ADH1 ACS2ND0.17 ± 0.050.23 ± 0.04
M8031Gpd Ba-adhE Eh-ADH1 ACS2 pADH1-ZWF1ND0.11 ± 0.034.49 ± 0.52
M9843Gpd Ba-adhE Eh-ADH1 ACS2 pHXT2-ZWF10.033 ± 0.0020.13 ± 0.020.71 ± 0.05
  • a Gpd, GPD1/GPD2 deletion; Ba-adhE, B. adolescentis adhE acetaldehyde dehydrogenase (acetylating), Eh-ADH1, E. histolytica ADH1.

  • b Values represent the average ± SD for duplicate cultures. NADPH-ADH, NADPH-specific alcohol dehydrogenase; ACS, acetyl-CoA synthetase; G6PDH, glucose-6-phosphate dehydrogenase.

  • c ND, not determined.