TABLE 1

Results of spiking spinach with STEC Sakaia

Sampling time and spike (CFU)E. coli phylogroup E (%)bMapped reads (%)cCoverage (fold)d
Preenrichment
    100.0030.010.04
    1,0000.0030.050.11
    100,0000.1190.030.10
    1,000,0000.3560.130.41
    10,000,000e4.2510.843.16
5-h enrichment
    10f0.0080.080.24
    1,000f0.0660.120.39
    10,000e7.4183.3013.3
    100,00030.44917.0970.9
23-h enrichment
    10f66.95449.73184
    1,000f84.33061.77252
  • a Samples of 100 g spinach were spiked with STEC Sakai.

  • b Percentage of the bacterial population belonging to E. coli phylogroup E.

  • c Percentage of total reads mapping to the STEC Sakai genome.

  • d Average chromosome coverage. The values were normalized to 10,000,000 total reads per sample. The coverage for the two plasmids was greater than that of the chromosome for all samples.

  • e Average of triplicate biological samples.

  • f Average of duplicate biological samples.