TABLE 1

Features of the ORFs of L. garvieae phage GE1b

ORFStrandaPositionSize (aa)MM (kDa)pIPutative RBS and start codoncPredicted functiondBest-match BLASTp resultNo. of aa shared with best match/total no. of aa in best match (% identity)E valueSizee (aa)Accession no.
StartStop
152910414116.96.8AGGAGActagaATG
2678526506.19.8aAGGAGtgcaagctATG
3854675597.29.3AAGAAAGgcaatgtagtgaaagaATG
41039842657.79.5AAGAAAGAggtagaaaATG
5129510298810.19.8AAGAAAGaaaacatctaaaATG
616501477576.88.7gAAGAGGtatagaaATG
7194916539811.14.5AGAGGAttgagATG
82256195110111.54.5GAGgtGAAaagagcATG
92678225314116.210.1AGGAGtttatagaccaGTGHNH endonucleaseORF31 Lactococcus phage 71266/137 (48)3e−32141YP_764291.1
10294726759010.47.9GAAAGGGGAtatatATG
113588307317120.09.4AGGAGAactttaATGORF41 Lactococcus phage 19154/162 (33)5e−11177AFE86775.1
1237703588607.14.7GAAAGGccattATGtRNA synthetaseORF6 Lactococcus phage c224/52 (46)0.1554NP_043532.1
134179375414116.49.8ctgacttgccattttaacATGHNH endonucleaseORF48 Lactococcus phage P47574/142 (52)3e−36145AGI11121.1
144510418110911.94.7AAGAGAatataaattATGssDNA-binding proteinORF40 Lactococcus phage 749/117 (42)2e−18119YP_008318242.1
155031453116618.37.8AGGAAAttgaattcgcATGERF recombinaseRec, S. pseudopneumoniae58/123 (47)1e−29207WP_000163473.1
1652315028677.85.2AGGAGGacaagaagaaATG
1754025268445.46.7atacAGAGGtggatagATG
18563753898210.09.5AtgAGGGAtttaagttagATGORF10 Lactococcus phage c231/77 (40)2e−0583NP_043536.1
19592056219911.94.9CGTAGAGGActttggcaagtATG
20617659138710.34.6AGGAGAgtaatATGgp25 Lactococcus phage KSY127/60 (45)3e−0799YP_001469023.1
2165316352597.17.9AGGttGAGAtattagaATG
227478652831636.15.4AAGGAGtAaatacaGTGDNA polymeraseORF42 Lactococcus phage jm3166/317 (52)6e−106315YP_008318192.1
23773774659010.58.9GGAAAGGctttggattATG
2481487921759.08.9AGGAGAaaacaaaATG
2583558218455.49.4AAAGGAcggtgttattATG
2684768342445.19.7GAGAGGtttagaaaATG
2786518466617.34.7AGGAGActattatATG
28+93099461505.84.5AGGAGAaaggtttATG
29+9463995116218.25.2tactcattacctatttaatATGHolinORF24 Lactococcus phage c292/159 (58)1e−59161NP_043550.1
30+104961116722324.99.0AAAGGAAAtaattaaTTG
31+111771210931034.75.2AAAtAGAGGtaacaaATG
32+120961366752357.64.9GAAGAGtgaggtagaagATGMCPMCP Lactococcus phage CB17130/535 (24)9e−22489AAZ95019.1
33+136841448426628.95.2AAAGAGgtaacttATG
34+144861510020422.44.7AAGAAAGGtataatttaatATGMajor tail proteinORF23 Lactococcus phage Q5465/200 (33)1e−21212YP_762592.1
35+1515115342636.76.0AGGGGGAAggTTGIg-like domain
36+1536615617839.65.6AGGGGActtaatATG
37+157981831783987.85.5AGGAAAttaatTTGTail proteinPl10 Lactococcus phage 5447123/471 (26)3e−22622AAT73602.1
38+18331186069110.29.3AGGGAAaataATGHolinGl17 Lactococcus phage 92354/85 (65)1e−2796AAT81366.1
39+18684189689410.98.3AGcttgtGGGGGtAtcATGHNH endonucleaseGp27 Lactococcus phage Q5444/101 (44)4e−16103YP_762596.1
40+189682060554561.36.6AtGGGGtGGtaATGTerminaseORF28 Lactococcus phage Q54191/507 (38)2e−96514YP_762597.1
41+20605209019811.49.1AGGGAAattaatctaATGORF35 Lactococcus phage c220/65 (31)0.00599NP_043561.1
42+209012210039946.65.5AAGGAGtttatgtaATG
43+221002275921924.95.8GAAAGGttacgggtataatttaATG
44+227592352625527.54.8GAAGGGttatgttggtggattaATG
452370823568464.99.5AGGAGAacatcATG
46242372379714616.410.2GAAAGGtggttatatgtaATG
472441024237576.64.0AAGcAGGttatataaaagATG
482457624394606.84.7AGGAAAtatattATG
  • a Orientation of the gene in the genome.

  • b Abbreviations: MM, molecular mass; RBS, ribosomal binding site; ssDNA, single-stranded DNA; MCP, major capsid protein; Rec, recombinase.

  • c Uppercase letters represent hypothetical RBS sequences (also underlined); bold letters represent starting codons.

  • d —, no significant matches.

  • e Total size of the aligned proteins.