TABLE 1

Strains and plasmids used in this study

NameDescriptionReference or source
P. aeruginosa PA14
    WTClinical isolate UCBPP-PA14, wild type59
    Δphz (LD24)ΔphzA1-G1 ΔphzA2-G2 deletion mutant60
    Δpel (LD82)ΔpelB-G deletion mutant5
    Δphz Δpel (LD83)ΔphzA1-G1 ΔphzA2-G2 ΔpelB-G deletion mutant5
    phz+ (DKN370)Contains two copies of phzM (pyocyanin overproducer)4
    pel+ (LD1471)ΔPpel + PA1/04/03 promoter swap mutant (constitutive Pel producer)This study
    eShak (LD1482)Strain isolated after three passages of shaken culture growthThis study
    ePel (LD1480)Strain isolated after three passages of pellicle growthThis study
    eColcenter (LD2243, LD2246 LD2249, LD2252)Evolved populations obtained after three passages of colony center growthThis study
    eColedge (LD2231, LD2234 LD2237, LD2240)Evolved populations obtained after three passages of colony edge growthThis study
    eColwhole (LD2255, LD2258, LD2261, LD2264)Evolved populations obtained after three passages of whole colony growthThis study
E. coli
    UQ950E. coli DH5α λ(pir) host for cloning; F Δ(argF-lac)169 φ80dlacZ58ΔM15 glnV44(AS) rfbD1 gyrA96(Nalr) recA1 endA1 spoT1 thi-1 hsdR17 deoR λpirD. Lies, Caltech
    BW29427thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir]W. Metcalf, University of Illinois
    ß2155thrB1004 pro thi strA hsdsS lacZΔM15 (F′ lacZΔM15 lacIq traD36 proA+ proB+) ΔdapA::erm (Ermr) pir::RP4 [::kan (Kmr) from SM10]61
S. cerevisiae
    InvSc1MATa/MATα leu2/leu2 trp1-289/trp1-289 ura3-52/ura3-52 his3-Δ1/his3-Δ1Invitrogen
Plasmids
    pMQ30sacB CEN/ARSH URA3+ Gmr, yeast-based vector , used for allelic exchange33
    pAKN69-eYFPContains mini-Tn7(Gm)PA1/04/03::eYFP fusion, used to genomically integrate constitutive YFP in PA14 strains34