TABLE 2

Primers used for generation of the pel+ mutant

PrimerSequence (5′ to 3′)
Upstream region flanking Ppel
    5′CCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATAGCAAGATCGACCTCAAGGA
    3′AAAAAGGGGACCTCTAGGGTCCGCCAGAATGAAAGAGCC
PA1/04/03
    5′GGCTCTTTCATTCTGGCGGACCCTAGAGGTCCCCTTTTT
    3′CCTTTCTTGCTGAACCGCATGCTTAATTTCTCCTCTTTAATTCTAGATGTG
Downstream region flanking Ppel
    5′CACATCTAGAATTAAAGAGGAGAAATTAAGCATGCGGTTCAGCAAGAAAGG
    3′GGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTGCAGGTAGTCGACCCGATAA