TABLE 1

Strains used in this study

Strain or plasmidRelevant genotypeaSource or reference
Strains
    Legionella pneumophila
        JR32 (WT)Philadelphia-1; Smr; r m+31
        LM1376 (rpoS)JR32 rpoS::Tn903dGent; Gmr25
        SPF176 (prpoS)LM1376(pSF49)This study
        KS79JR32 ΔcomR78
        SPF197 (relA)KS79 relA::aacC1; GmrThis study
        SPF204 (relA spoT)SPF197 spoT::aptII; Gmr KmrThis study
    Escherichia coli
        CF1648 (MG1655)F λ ΔilvG rfb-50 rph-134
        CF1693 (MG1655 relA spoT)ΔrelA251 ΔspoT20734
Plasmids
    pMMB207cCmr; ΔmobA79
    pSF49rpoS in pMMB207cThis study
  • a Cmr, chloramphenicol resistance; Gmr, gentamicin resistance; Smr, streptomycin resistance; Kmr, kanamycin resistance.