TABLE 2

Plasmids and B. burgdorferi strains used in this study

Plasmid or strainResistanceaDescription
E. coliB. burgdorferi
Plasmids
    pOK-bbe02KanamycinPlasmid harboring partial bbe02 gene
    pKBE-StrepSpectinomycin and kanamycinStreptomycinbbe02 allelic-exchange vector, MCS, and flaBp-aadA
    pKBE-KanKanamycinKanamycinbbe02 allelic-exchange vector, MCS, and flgBp-aphI
    pKBE-Strep/ospC+Spectinomycin and kanamycinStreptomycinpKBE-Strep with native ospC cloned in the MCS
B. burgdorferi strain
    B31-A3NoneWT strain B31 infectious clone (3)
    ospCK1KanamycinB31-A3 derivative with deletion of ospC gene (19)
    ospCK1/pBSV2G-ospCGentamicin and kanamycinospCK1 derivative with ospC on shuttle vector (19)
    ospCK1/ospC+KanamycinospCK1 with deletion in bbe02 and insertion of ospC
    A3 Δbbe02-StreprStreptomycinB31-A3 with deletion in bbe02 and insertion of flaBp-aadA
    A3 Δbbe02-KanrKanamycinB31-A3 with deletion in bbe02 and insertion of flgBp-aphI
  • a Antibiotic resistance conferred by plasmids in E. coli or B. burgdorferi or exhibited by the strain.