TABLE 1

Primers and probes used for detection of functional genes in E. coli isolates

GeneFunctionPrimer/probe nameSequenceSize (bp)
sfmHFimbrial-like adhesion proteinsfmH.F5′-TGCGTCTGGAAGCCAGTGCC-3′220
sfmH.R5′-GCGCTAAACGGCCCYTCGGT-3′
sfmH.P5′-GTCTGGATGCAGCTGCGGCAG-3′
rfaILipopolysaccharide biosynthesis proteinrfaI.F5′-TGCTTGGCGCAATAACGAGCA-3′142
rfaI.R5′-TCGGCCCGACAAAACCCTGG-3′
rfaI.P5′-TGCGCRCTTGGATAACCGGCCCAGTA-3′
fucKSugar kinases for 5-C and 6-C sugarsfucK.F5′-ACAGAACAGCGCCGCAGCAA-3′162
fucK.R5′-ACCTGGGCGCTRGGACCAT-3′
fucK.P5′-CGCCCTTTTTGGCGCTGGTGCCG-3′