TABLE 2

Lysine decarboxylase activity of clones from acid-evolved populationsa

No. of generationsNo. of isolates in the following population testing negative for lysine decarboxylase activity:
A7B7C7D7E7F7G7H7A9B9C9D9E9F9G9H9A11B11C11D11E11F11G11H11
7601
1,400123211223123111
2,000333333333333333323333333
  • a For each generation, three isolates from each population were assayed. The number of isolates of three isolates tested that lost lysine decarboxylase activity (indicated by a yellow color in Moeller broth) after 24 h of culture in a microplate is indicated.