TABLE 4

Intergenic mutations identified in the 2,000th generation acid-adapted clonesa

Proximal gene(s)Mutation (positions, nucleotide change)Identification of a mutation in clone:
H9-1H9-2B11-1B11-2F11-1F11-2F9-2F9-3
matP →/← ompAIntergenic (+40/+36, C to A)X
racR ←/→ ydaSIntergenic (−61/−63, C to A)X
Y75_p4289 ←/→ eaeHIntergenic (−545/−1035, C to A)X
proLNoncoding (57/77 nt, C to A)XX
nuoA ←/← lrhAIntergenic (−87/+544, C to A)XX
yfjL ←/← yfjMIntergenic (−258/+102) +8 bp (GCACTATG)X
rhaA →/→ rhaDIntergenic (+308/−143, C to A)XX
rrsCNoncoding (1417/1542 nt, +A)XX
pabA →/→ argDIntergenic (+34/−52, A to G)XX
yjbI →/→ ubiCIntergenic (+84/−139, G to T)XX
dcuA ←/← aspAIntergenic (−202/+104, G to T)XX
acs ←/→ nrfAIntergenic (−228/−165, G to A)XX
  • a The mutations listed indicate intergenic differences between the adapted clones and the ancestral wild type. A positive distance from a proximal gene represents the downstream location of the mutation relative to the gene's translation stop site, whereas a negative distance represents the mutation's upstream location relative to the gene's translation start site. nt, nucleotide position number.