TABLE 1

Strains and plasmids used in this study

Strain/plasmidRelevant characteristicSource/reference
E. coli JM109Cloning strain
E. coli S17-1Conjugation strain67
R. eutropha H16R. eutropha wild-type strainDSMZ 428
H16ΔA0671Chromosomal deletion of A0671This study
H16ΔB1632Chromosomal deletion of B1632This study
H16ΔA0671ΔB1632Chromosomal deletions of A0671 and B1632This study
pBBR1MCS2Broad-host-range vector; Kmr68
pBBR1MCS2-PphaC-eyfp-c1Universal vector for construction of fusions C-terminal to eYFP under the control of the PphaC promoter43
pBBR1MCS-2-PphaC-eyfp-A0225N-terminal fusion of A0225 to eYFPThis study
pBBR1MCS-2-PphaC-eyfp-A0671N-terminal fusion of A0671 to eYFPThis study
pBBR1MCS-2-PphaC-eyfp-A2001N-terminal fusion of A2001 to eYFPThis study
pBBR1MCS-2-PphaC-eyfp-B1632N-terminal fusion of B1632 to eYFPThis study
pBBR1MCS-2-PphaC-eyfp-phaZcN-terminal fusion of PhaZc to eYFPThis study
pBBR1MCS-2-PphaC-eyfp-A3253N-terminal fusion of A3253 to eYFPThis study
pBBR1MCS-2-PphaC-eyfp-A2924N-terminal fusion of A2924 to eYFPThis study
pBBR1MCS-2-PphaC-eyfp-B0232N-terminal fusion of B0232 to eYFPThis study
pLO3Suicide vector; Tcr69
pLO3-ΔA0671Deletion vector for A0671; fragments up- and downstream of A0671 cloned between SacI and XbaI sites of pLO3This study
pLO3-ΔB1632Deletion vector for B1632; fragments up- and downstream of B1632 cloned between SacI and XbaI sites of pLO3This study