TABLE 2

Abundances of PGAPs

Granule rankaAll protein rankbLocuscPGAPdMass (kDa)AbundanceFold change (NB/MM)
NBMM
11A1381PhaP1201.6 × 10109.5 × 10817
22PHG202PhaP2205.2 × 1084.9 × 10711
37B2021PhaP4207.5 × 1077.9 × 1070.95
49B1632α/β-Hydrolase867.0 × 1079.9 × 1067.1
510A2172PhaP3206.1 × 1076.8 × 1070.90
611A1437PhaC1644.9 × 1071.9 × 1072.6
717B1934PhaP5163.1 × 1071.5 × 10621
818A1150PhaZa1472.4 × 1073.2 × 1067.5
928A0671α/β-Hydrolase841.3 × 1073.9 × 1063.3
1036A0225Phospholipase511.0 × 1071.3 × 1067.7
1140A2001Hypothetical protein189.2 × 1063.9 × 1062.4
12136B2326PhaP7161.5 × 1062.2 × 1056.8
13323B1988PhaP6232.9 × 1056.4 × 1044.5
14332A2003PhaC2652.6 × 1051.3 × 1052.0
1521A1440PhaR211.8 × 1073.4 × 1065.3
16224A0141PhaM275.8 × 1051.1 × 1055.3
22PHG252Nitrous oxide red701.4 × 1072.4 × 1070.58
29A0472ABC transporter338.2 × 1061.7 × 1070.48
30A3284Omp401.0 × 1073.1 × 1070.32
37A3030ABC transporter417.3 × 1062.9 × 1070.25
  • a Rank numbers of PHB granule-associated proteins by their relative abundances in granules of NB-grown cells (averages of three replicates).

  • b Rank numbers sorted by relative abundances of all proteins detected in PHB granules of NB-grown cells.

  • c Italics indicates examples of proteins that had higher abundance in PHB granules of fructose mineral salts medium-grown cells. Boldface indicates novel, specifically PHB granule-associated proteins.

  • d Note that PhaP1, PhaC1, PhaR, and PhaM are also present in other fractions.