TABLE 2

Sequences of oligonucleotide primers

NameSequence (5′–3′)Function and relevant characteristic(s)a
gapA_Del_AGGCTGATCCTCAAATGACCAAGDel of gapA
gapA_Del_BCCCATCCACTAAACTTAAACAACCAACACGAATGGTCATGTTGDel of gapA
gapA_Del_CTGTTTAAGTTTAGTGGATGGGCTGCGTCTGACCGAGCTCGTAGDel of gapA
gapA_Del_DCACCGAAGCCGTCAGAAACGAATGDel of gapA
gapA_Del_SeqGTTCGTTCCCTGCAAAAACTATTTAGDel of gapA
gapA_Del_Ver_fwCCAACTTCGACGATGCCAATCVerification of gapB deletion
gapA_Del_Ver_rvCTCTGGTGATTCTGCGATCTTTTCVerification of gapB deletion
gapB_Del_AGACGGTGACCAATCCGGAGDel of gapB
gapB_Del_BCCCATCCACTAAACTTAAACACTTGTGGTTGTGCGTCATAAAAAGTDel of gapB
gapB_Del_CTGTTTAAGTTTAGTGGATGGGGTGTACCCGGAGCGCAGGCAGDel of gapB
gapB_Del_DGCCACAATATTGGCTTTGAGGTTGDel of gapB
gapB_Del_SeqCTCACTTAACCGCGAGATCTTGGACDel of gapB
gapB_Del_Ver_fwGATTTGAGCAATGGGTGGGAGVerification of gapB deletion
gapB_Del_Ver_rvGATGACAGTGCACGATCATCATGVerification of gapB deletion
gap_Cacet_fwGATCTAGAGAAAGGAGGCCCTTCAGATGTTTGAAAATATATCATCAAATGGAGTTTATAAAAATCOE of C. acetobutylicum gapN; start RBS
gap_Cacet_rvGATCTAGATTATAGGTTTAAAACTATTGATTTATGCCTTGTCOE of C. acetobutylicum gapN
ndh-fwCTGCAGGTCGACTCTAGAGGAAAGGAGGCCCTTCAGATGTCAGTTAACCCAACCCGOE of ndh
ndh-revCGGTACCCGGGGATCTTACTTTCCGCTGAAACGCTGOE of ndh
udhA_fwGCTCTAGAGAAAGGAGGCCCTTCAGATGCCACATTCCTACGATTACGOE of udhA
udhA_revGCTCTAGATTAAAACAGGCGGTTTAAACCGOE of udhA
  • a Abbreviations: OE, overexpression; Del, deletion; RBS, ribosomal binding site.