Day | Total no. of cells^{a}/ml | Mean cell-cell distance^{b} (μm) | Maximum avg interspecies distance for H_{2} transfer^{c} (μm) |
---|---|---|---|

2 | 6.2 × 10^{6} | 55.3 | 14 |

4 | 5.0 × 10^{7} | 27.1^{d} | 6.3^{d} |

6 | 9.2 × 10^{7} | 22.2 | 2.7 |

8 | 13.7 × 10^{7} | 19.6 | 1.9 |

↵a That is, for strain 195 plus S. wolfei.

↵b The cell-cell distance was based on one sample and was calculated for suspended cells using the cell number quantified from quantitative PCR analysis.

↵c That is, for the transfer of S. wolfei to strain 195 at the observed substrate oxidation rate. Values were calculated using Fick's diffusion law.

↵d Detailed calculations for these values can be found in the supplemental material, part 4.