TABLE 1

Target, primer/probe sequences, and amplification conditions for endpoint PCR and qPCR assays used in this study

AssaysTarget genePrimer or probe sequence (5′–3′)aAmplification conditionsReference
Sketa22 endpoint PCRITS region 2F, GGT TTC CGC AGC TGG G10 min at 95°C, followed by 40 cycles of 15 s at 95°C and 45 s at 63°C54
R, CCG AGC CGT CCT GGT CTA
P, FAM-AGT CGC AGG CGG CCA CCG T-TAMRA
HAdV qPCRHexon geneF, GCC ACG GTG GGG TTT CTA AAC TT10 min at 95°C, followed by 40 cycles of 15 s at 95°C, 20 s at 60°C, and 20 s at 95°C55
R: GCC CCA GTG GTC TTA CAT GCA CAT C
HPyV qPCRHomologous T antigenF, AGT CTT TAG GGT CTT CTA CCT TT10 min at 95°C, followed by 40 cycles of 15 s at 95°C, 15 s at 55°C, and 60 s at 60°C19
R, GGT GCC AAC CTA TGG AAC AG
P, FAM-AGT CGC AGG CGG CCA CCG T-MGBNFQ
  • a F, forward primer; R, reverse primer; P, probe; FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine; MGBNFQ, molecular-groove binding nonfluorescence quencher.