TABLE 2

O. keta endpoint PCR assay for the evaluation of inhibition in raw sewage spiked into tap (n = 3) and river (n = 3) water nucleic acid samples as opposed to DNase- and RNase-free water samplesa

MethodbSample typeng of DNA/μl of extract (range)Mean CT ± SD for O. keta endpoint PCR assay
Undiluted nucleic acid10-fold-diluted nucleic acid
ATap water1.1–2.130 ± 0.4
River water3.3–5.829 ± 0.5
BTap water0.4–1.730 ± 0.3
River water6.5–17NAc30 ± 0.6
CTap water3.3–9.729 ± 0.3
River water4.6–30NA30 ± 0.7
DNase- and RNase-free water29 ± 0.1
  • a DNase- and RNase-free water samples and diluted and undiluted DNA samples were spiked with 10 pg of O. keta.

  • b A, the Mo Bio PowerMax soil DNA isolation kit was used to extract nucleic acid; B, a Qiagen DNeasy blood and tissue kit was used to extract nucleic acid; C, not tested.

  • c NA, no amplification.