TABLE 2

Primers and probes sequences used in this study for mRNA measurementsa

Target mRNAPrimer or probeSequence (5′–3′)
TBPFWTTGTACCGCAGCTTCAAAATATTGT
RVGCAGCAAATCGCTTGGGAT
TPFAM-TTGACCTAAAGACCATTGCACTTCGTGCA-TAMRA
B2MFWCCACTGAGACTGATACATACGCCT
RVGATCACATGTCTCGATCCCAGTAG
TPFAM-TAAGCATGCCAGTATGGCCGAGCC-TAMRA
SDHAFWACATCAGAACTACGCCTAAACATGC
RVAAACCATTCCCCTGTCGAATG
TPFAM-AACCATGCTGCAGTGTTCCGTGTGG-TAMRA
RPL13AFWGCGCCTCAAGGTGTTGGAT
RVCCCAGGTAAGCAAACTTTCTGG
TPFAM-TGGTCCCTGCTGCTCTCAAGGTTGTT-TAMRA
HPRTFWTTATCAGACTGAAGAGCTACTGTAATGATC
RVTTACCAGTGTCAATTATATCTTCAACAATC
TPFAM-TGAGAGATCATCTCCACCAATAACTTTTATGTCCC-TAMRA
PPIAFWGCCGCGTCTCCTTCGAG
RVGTAAAGTCACCACCCTGGCAC
TPFAM-TTGCAGACAAAGTTCCAAAGACAGCAGAAA-TAMRA
β-ActinFWAGAGGGAAATCGTGCGTGAC
RVCAATAGTGATGACCTGGCCGT
TPFAM-CACTGCCGCATCCTCTTCCTCCC-TAMRA
GAPDHFWTCACCACCATGGAGAAGGC
RVGCTAAGCAGTTGGTGGTGCA
TPFAM-ATGCCCCCATGTTTGTGATGGGTGT-TAMRA
IL-6FWGAGGATACCACTCCCAACAGACC
RVAAGTGCATCATCGTTGTTCATACA
TPFAM-CAGAATTGCCATTGCACAACTCTTTTCTCA-TAMRA
IL-10FWGGTTGCCAAGCCTTATCGGA
RVACCTGCTCCACTGCCTTGCT
TPFAM-TGAGGCGCTGTCATCGATTTCTCCC-TAMRA
  • a TBP, TATAA box binding protein; B2M, β2-microglobulin; SDHA, succinate dehydrogenase complex subunit A; RPL13A, ribosomal protein L13a; HPRT, hypoxanthine phosphoribosyltransferase; PPIA, peptidyl-prolyl cis-trans-isomerase A; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-6, interleukin-6; IL-10, interleukin-10; FW, forward primer; RV, reverse primer; TP, hydrolysis probe; FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.