TABLE 1

Strains and plasmids used in this study

Strain or plasmidRelevant characteristicsSource or reference
E. coli DH5αF ϕ80dlacΔ(lacZ)M15 Δ(lacZYA-argF) U169 endA1 recA1 hsdR17(rK mK+) deoR thi-1 phoA supE44 λ gyrA96 relA1Invitrogen (Karlsruhe, Germany)
B. subtilis 168Wild type, trpC2, auxotrophic for tryptophanBGSCa
C. glutamicum strains
    ATCC 13032Biotin auxotroph, wild-type strain36
    Δald ΔadhEDerivative of ATCC 13032 with in-frame deletion of ald (cg3096) and adhE (cg0387)22
Plasmids
    pEKEx2Kanr; C. glutamicum-E. coli shuttle vector for regulated gene expression; Ptac, lacIq pBL1 oriVCg pUC18 oriVEc37
    pVWEx2Tetr; C. glutamicum-E. coli shuttle vector for regulated gene expression; Ptac, lacIq pCG1 oriVCg pUC18 oriVEc38
    pVWEx2-Bm(mdh-act)Tetr; pVWEx2 derivative containing Bm(mdh-act) under control of PtacThis work
    pVWEx2-Ptuf-Bm(mdh-act)Tetr; pVWEx2 derivative containing Bm(mdh-act) under control of PtufThis work
    pVWEx2-Bm(mdh3-act)Tetr; pVWEx2 derivative containing Bm(mdh3-act) under control of PtacThis work
    pVWEx2-Ptuf-Bm(mdh3-act)Tetr; pVWEx2 derivative containing Bm(mdh3-act) under control of PtufThis work
    pVWEx2-CgadhATetr; pVWEx2 derivative containing CgadhA under control of PtacThis work
    pEKEx2-Bs(hps-phi)Kanr; pEKEx2 derivative containing Bs(hps-phi) under control of PtacThis work
    pEKEx2- Ptuf -Bs(hps-phi)Kanr; pEKEx2 derivative containing Bs(hps-phi) under control of PtufThis work
    pEKEx2-Mg(hps-phi)Kanr; pEKEx2 derivative containing Mg(hps-phi) under control of PtacThis work
  • a BGSC, Bacillus Genetic Stock Center.