TABLE 3

Genetic engineering of S. epidermidis using phage Φ187-mediated plasmid transfer

Bacterial strainCounterselection efficiency (%)asigB mutagenesis efficiency (%)b
S. epidermidis 33b10085.7
S. epidermidis IVK8396.442.8
  • a Counterselection efficiency was calculated after anhydrotetracycline selection by dividing the number of chloramphenicol-sensitive clones by the total number of tested isolates.

  • b Mutagenesis efficiency was calculated after PCR analysis by dividing the number of sigB deletion mutants by the total number of tested isolates.