TABLE 2

Artificial gfp genes, plasmids, host strains, cell lines, phages, and virus used in this study

Gene, plasmid, host strain, cell line, phage or virusAccession no.Description
Genes
    gfpJC175864The original gfpmut3 gene (2)
    lgfpLC018331The gfpmut3 gene with all codons changed to those with the lowest usage in E. coli
    lgfpΔ1LC018332The modified lgfp gene with 180 bp (25%) deleted from C terminus
    lgfpΔ2LC018333The modified lgfp gene with 357 bp (50%) deleted from C terminus
    lgfpΔ3LC018334The modified lgfp gene with 537 bp (75%) deleted from C terminus
    hgfpLC018335The gfpmut3 gene with all codons changed to those with the highest usage in E. coli
    hgfpΔ1LC018336The modified hgfp gene with 180 bp (25%) deleted from C terminus
    hu-lgfpLC018337The AcGFP1 gene with all codons changed to those with the lowest usage in H. sapiens
Plasmids
    pLGFPLC018338IPTG-inducible lgfp, ColE1 origin, Ampr
    pHGFPLC018344IPTG-inducible hgfp, ColE1 origin, Ampr
    pLGFPmLC018342IPTG-inducible lgfp, p15A origin, Ampr
    pLGFPlLC018342IPTG-inducible lgfp, SC101 origin, Ampr
    pLGFPΔ1LC018339IPTG-inducible lgfpΔ1, ColE1 origin, Ampr
    pLGFPΔ2LC018340IPTG-inducible lgfpΔ2, ColE1 origin, Ampr
    pLGFPΔ3LC018341IPTG-inducible lgfpΔ3, ColE1 origin, Ampr
    pLΔNGLC018348IPTG-inducible lgfpΔ1, aTc-inducible gfp, ColE1 origin, Ampr
    pHΔNGLC018349IPTG-inducible hgfpΔ1, aTc-inducible gfp, ColE1 origin, Ampr
    pPALLC018350f1 phage infection-inducible lgfp, ColE1 origin, Ampr
    pPAHLC018351f1 phage infection-inducible hgfp, ColE1 origin, Ampr
    pRARENAaCarries rare-codon tRNAs, p15A origin, chloramphenicol resistance
    pYEGLC018345Galactose-inducible yEGFP in S. cerevisiae, aureobasidin A resistance, CEN/ARS
    pYLGFP1LC018346Galactose-inducible lgfp in S. cerevisiae, aureobasidin A resistance, CEN/ARS
    pYLGFP2LC018347Galactose-inducible hgfp in S. cerevisiae, aureobasidin A resistance, CEN/ARS
    pTRE-LucNADox-inducible luciferase in mammalian cells, G418 resistance
    pTRE-G1LC018352Dox-inducible hu-lgfp in mammalian cells, G418 resistance
Host strains
    E. coli
        Mach1F ϕ80(lacZ)ΔM15 ΔlacX74 hsdR(rK mK+) ΔrecA1398 endA1 tonA
        XL-10deoR endA1 gyrA96 hsdR17(rK mK+) recA1 relA1 supE44 thi-1 Δ(lacZYA-argF)U169 ϕ80dlacZΔM15 F λ PN25/tetR placIq/lacI
        JM2.300lacI22 LAM e14 rpsL135 (Strr) xyl-7 mtl-1 thi-1
        AK4λ lacI::Km mutant of K-12 (CGSC7296) wild-type strain
    S. cerevisiae YPH499MATα his3200 leu21 lys2-801 trp11 ade2-101 ura3-52
Human cell lines
    HeLa-Tet-ONHeLa cells optimized for Tet-ON gene expression system
    MCF7-Tet-ONMCF7 cells optimized for Tet-ON gene expression system
    HEK293-Tet-ONHEK293 cells optimized for Tet-ON gene expression system, host of recombinant adenovirus
Phages
    T4Enterobacterial phage T4, linear double-stranded DNA phage
    T7Bacteriophage T7, linear double-stranded DNA phage
    λEnterobacterial phage l, linear double-stranded DNA temperate phage
    f1Enterobacterial phage f1, a filamentous single-stranded DNA phage
    MS2Bacteriophage MS2, an icosahedral linear single-stranded RNA+ phage
Recombinant adenovirusA recombinant virus vector for gene transfer, no gene was coded in this expt
  • a NA, not available.