TABLE 3

Estimated plasmid copy numbers of S. aureus RN4220 transformants determined by quantitative real-time PCR

PlasmidCT (avg ± SD)aAvg no. of copies (CV [%])bPCN (avg ± SD)c
nuctet(L)nuctet(L)
None21.44 ± 0.033.12 × 105 (1.8)
pBUS121.52 ± 0.0620.59 ± 0.112.97 × 105 (3.4)3.71 × 106 (6.9)12.48 ± 0.86
pBUS1-Pcap22.04 ± 0.0920.48 ± 0.102.16 × 105 (5.4)3.98 × 106 (6.4)18.45 ± 1.18
pBUS1-HC21.56 ± 0.0220.15 ± 0.032.91 × 105 (1.4)4.87 × 106 (1.9)16.74 ± 0.31
pBUS1-Pcap-HC21.28 ± 0.0519.55 ± 0.073.45 × 105 (3.3)7.12 × 106 (4.2)20.60 ± 0.86
pTSSC21.00 ± 0.0519.20 ± 0.024.12 × 105 (2.8)8.81 × 106 (1.5)21.41 ± 0.32
pTSSC-Pcap20.77 ± 0.0618.55 ± 0.104.73 × 105 (3.9)1.32 × 107 (6.1)27.93 ± 1.71
pTSSCm20.98 ± 0.0219.18 ± 0.034.17 × 105 (1.3)8.91 × 106 (2.1)21.39 ± 0.44
pTSSCm-Pcap21.25 ± 0.0219.06 ± 0.063.52 × 105 (1.2)9.59 × 106 (4.0)27.27 ± 1.06
  • a n = 3.

  • b CV, coefficient of variation (n = 3).

  • c PCN, plasmid copy number (n = 3).