TABLE 2

Quantitative PCR of host-specific Wolbachia variants

GeneSlopePCR efficiencyDilutionCT for indicated host-strain combination
Dmel wMelDmel wMelCSDsim wMelDsim wRi
Reference wsp gene−3.1082.09771:1019.1521.7121.4621.55
−3.1082.09771:10022.2925.0524.4824.86
Target WD0513 gene−3.0852.10941:1019.2121.9721.832.97
−3.0852.10941:10022.2624.4924.5335.25
Fold change relative to control1:101.000.850.800.00
1:1001.001.460.930.00
    Mean (SE)1.00 (±0.0)1.16 (±0.43)0.87 (±0.09)0.00 (±0.00)