TABLE 2

Numbers of transformants and mutations obtained with the various CRISPR-Cas9 plasmids

PlasmidNo. of transformants/μg% kanamycin-resistant colonies (total no. of colonies tested)a% ΔamyE or trpC+ mutants (total no. of colonies tested)% of PCR fragments of expected size (total no. of colonies tested)
pJOE9012.11,033 ± 2466.9 (72)93.9 (72)89 (47)
pJOE9150.21,222 ± 37915.2 (46)97 (46)
pJOE9198.11,206 ± 3682.7 (72)100 (72)100 (44)
pJOE9202.12,167 ± 5291.5 (66)22.9 (66)100 (24)
pWAL2751,594 ± 575
  • a Colonies were tested after growth at 50°C and 42°C.