TABLE 1

Strains, plasmids, and primers used in this study

Strain, plasmid, or primerDescriptionaSource or reference
E. coli strains
    TOP10F recA1 endA1 hsdR17(rK mK+) supE44 thi-1 gyrA relA1Invitrogen
    GM2163F ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2New England Biolabs
H. volcanii strains
    DS70Wild-type isolate DS2 cured of plasmid pHV264
    H26DS70 Δ pyrE265
Plasmids
    pJAM503Apr Nvr; H. volcanii-E. coli shuttle vector with coding sequence for N-terminal His6 tag29
    pJAM2920Apr Nvr; pJAM503 derived, His6-HvPPAThis study
Primers
    1, PPA NdeI FW5′-tacatatgGTGAACCTCTGGGAAGATATGGAG-3′This study
    2, PPA BlpI RV5′-tagctcagctcgcTTACGCGAAGTTCTCTTCGTAG-3′This study
  • a Apr, ampicillin resistance; Nvr, novobiocin resistance. Underlining and lowercase in the primer sequences indicate restriction sites and linkers/overhangs, respectively.