TABLE 2

Purification of HvPPA from Haloferax volcaniia

FractionProtein (mg)Total activity, U (mean ± SD)Sp act, U · mg−1 (mean ± SD)Approx yield, %Purification, fold enrichment
Lysate1,680356 ± 300.21 ± 0.021001
HisTrap HP23180 ± 148.0 ± 0.65138
Superdex 2003.0204 ± 0.368 ± 0.557322
  • a Based on standard purification from 4 1-liter cultures of H. volcanii strain H26-pJAM2920. PPA activity was monitored in Tris-salt buffer at 37°C and pH 8.5 with 0.1 mM PPi for 10 min. Units are defined as micromoles of product · minute−1. Values are for three experiments.