TABLE 1

Different bacterial expression strains and plasmids used in the expression study to determine the strain yielding recombinant PgtCDA with the highest yield and highest specific activity

Construct no.Expression strainPlasmidProtein yield (nmol/ml)Enzyme activity (nkat/ml)Specific activity (nkat/nmol)
CDA1Rosetta disulfide/pLysSRARE/pCDFDuet1-DsbC/TrxApET22b::PgtCDA-Strep IIC3.0821
CDA2Rosetta2(DE3)/pLysSRAREpET22b::PgtCDA-Strep IIC2.265825
CDA3BL21(DE3)pET22b::PgtCDA-Strep IIC2.4520282
CDA4Rosetta disulfide/pLysSRARE/pCDFDuet1-DsbC/TrxApET22b::MBP-PgtCDA-Strep IIC4.38719164
CDA5Rosetta2(DE3)/pLysSRAREpET22b::MBP- PgtCDA-Strep IIC4.851,001206
CDA6BL21(DE3)pET22b::MBP-PgtCDA-Strep IIC6.571,700258
CDA7ER2508pMAL-p5x::MBP-PgtCDA-Strep IIC1.70697410
CDA8Rosetta2(DE3)/pLysSRAREpMAL-p5x::MBP-PgtCDA-Strep IIC3.301,485450