TABLE 1

Oligonucleotide primers

TABLE 1
  • a The restriction sites XhoI (CTCGAG, for forward expression primers) and NotI (GCGGCCGC, for reverse expression primers [only partial in the GtLPMO9A-2 primer]) and the sequence encoding the cleavage site of Kex2 (AAAAGA, encoding Lys-Arg in forward primers) are printed in italics. ORF sequences are underlined. Expression primers were designed with a protecting AA sequence at the 5′ end. GtLPMO9A-2 expression primers, for In-Fusion cloning, were designed to contain 15 extra bases at the 5′ end, overlapping the Kex2 (forward) or NotI restriction cleavage site (reverse) of pPICZα.