TABLE 2

Parameters obtained after fitting the dynamic viscosity curvesa

SubstrateEnzymeFitParameterR2bIDR (mP · ks−1)c
a (mP)b (ks−1)c (mP)
XGGtLPMO9A-2Exp.0.5748−0.15140.98120.99270.0870
NcLPMO9CExp.0.4633−0.10670.99470.99500.0495
GMGtLPMO9A-2Exp.0.7115−0.074161.04960.99660.0528
NcLPMO9CExp.0.3907−0.15370.93670.98320.0600
WAXGtLPMO9A-2Lin.−0.0014541.71060.99360.001454
NcLPMO9CLin.−0.0016541.6630.97310.001653
CMCGtLPMO9A-2Exp.0.3713−0.019991.70820.99980.0074
NcLPMO9CExp.0.4455−0.027321.66610.99910.0122
  • a The correlation between dynamic viscosity and time (data from Fig. 4>) was fitted to an exponential (Exp.) curve (y = a × e[b × x] + c) or to a linear (Lin.) equation (y = a × x + b), where y is the dynamic viscosity (in millipoise), x is the time (in kiloseconds), and a, b, and c are constants. The constant c is the final viscosity of substrate after completion of LPMO9 action.

  • b The high R2 values (>0.97) reflect a good fit of the model.

  • c The initial depolymerization rate (IDR) was calculated as the first derivative for the formula at x = 0.