TABLE 2

Soluble proteins identified from L. biflexa during exponential phasea

SpotDescription (Uniprot accession no.)% coverageNo. of unique peptidesMass (kDa)bpIb
1*Acyl carrier protein (B0SDK7)65148.503.99
250S ribosomal protein L7/L12 (B0SAG2)9111812.655.20
3*Thioredoxin (B0SBX2)837411.415.04
5*Nucleoside diphosphate kinase (B0S9J3)845715.286.90
6*Acyl dehydratase MaoC family (B0SGL8)9411314.956.83
7*Uncharacterized protein (B0SGL7)947016.955.75
8Peptidyl-prolyl cis-trans isomerase (B0SGI0)686018.134.91
9ATP-dependent Clp protease proteolytic subunit (B0S8T0)613222.424.92
10*Adenylate kinase (B0SA26)8810720.495.52
12Superoxide dismutase (B0S932)581021.296.22
13Elongation factor Ts (B0SDN6)778321.836.23
14Hydroxyethylthiazole kinase (B0SE82)7312627.616.83
15Short-chain dehydrogenase (B0SDL1)8212826.267.69
16Short-chain dehydrogenase (B0S953)9312227.018.25
17*Electron transfer flavoprotein alpha subunit (B0SF29)8820832.728.63
19*Spermidine synthase (B0SI93)869431.825.87
20*Enoyl-CoA hydratase/isomerase family protein (B0SDX5)8011127.355.53
21Electron transfer flavoprotein beta subunit (B0SF30)8221127.325.34
22*Elongation factor P (B0SII2)786921.304.93
23Uncharacterized protein (B0SE41)8827139.194.95
24DNA-directed RNA polymerase subunit alpha, RpoA (B0SA20)8816336.604.86
25Acyl-CoA dehydrogenase (B0S960)7716142.325.75
26*Ketol-acid reductoisomerase (B0SCQ5)7710736.166.03
27Citrate lyase beta subunit (B0SCW2)451637.076.10
28Malate dehydrogenase (B0SF41)511534.896.25
29*Succinyl-CoA ligase [ADP-forming] subunit alpha (B0SAP1)26630.546.66
Uncharacterized protein (B0SHJ5)581531.508.26
30Aldolase (B0SGS2)611838.407.71
31Aspartate-semialdehyde dehydrogenase (B0SIQ0)441537.807.09
Glyceraldehyde 3-phosphate dehydrogenase (B0SB20)521436.506.55
32Glyceraldehyde 3-phosphate dehydrogenase (B0SB20)903736.506.55
34Acyl dehydratase (B0SD87)843044.006.77
35*Acyl-CoA dehydrogenase (B0SF91)411142.526.43
Glyceraldehyde 3-phosphate dehydrogenase (B0SB20)47936.446.55
36S-Malonyltransferase [acyl carrier protein] (B0SA97)742343.115.35
37ATP synthase subunit beta (B0SDA5)632251.075.28
38Acetyl-CoA acetyltransferase (B0SCQ4)742547.265.66
39ATP synthase subunit alpha (B0SDA3)673455.006.05
40Uncharacterized protein (B0SCE1)575163.207.97
41Aconitate hydratase (B0SIM5)553581.106.25
42Polyribonucleotide nucleotidyltransferase (B0SH22)594175.905.85
43Phosphoglycerate mutase (B0SBJ6)492061.405.66
4460-kDa chaperonin GroEL (B0SCC0)774858.105.35
45Trigger factor (B0SME8)c803650.705.19
46Chaperone protein DnaK (B0SHT1)523468.995.04
47Elongation factor G (B0SF49)633678.805.39
48*Methionine synthase (B0SIG4)3133137.585.40
49Uncharacterized protein (B0SH48)46819.418.95
51Phosphoribosylformylglycinamidine cyclo-ligase (B0SDW2)641836.715.38
52*Thioredoxin reductase (B0SCD5)38833.666.19
53S-Adenosylmethionine synthase (B0SFH3)552342.206.13
543-Hydroxyacyl-CoA dehydrogenase (B0SC49)612547.638.47
55*Acetyl-CoA carboxylase, carboxyltransferase component subunits alpha and beta (B0SHH5)351759.866.14
  • a Proteins were identified by LC-MS/MS on two independently isolated samples except for those marked with an asterisk, which indicates only a single LC-MS/MS identification. Numbers provided are from a single, representative run. CoA, coenzyme A.

  • b Calculated.

  • c Identified only in the L. biflexa Paris strain; no hit was obtained against the Ames strain.