TABLE 3

Membrane-associated proteins identified during exponential phasea

SpotDescription (Uniprot accession no.)% coverageNo. of unique peptidesMass (kDa)bpIb
10050S ribosomal protein L7/L12 (B0SAG2)969212.655.20
101*30S ribosomal protein S6 (B0SB40)912710.436.29
103OmpA-family lipoprotein (B0SGK2)8511420.616.62
104*LipL21 lipoprotein (B0S915)514220.417.98
OmpA-family lipoprotein (B0SGK2)712220.616.62
105ATP synthase subunit delta (B0SDA2)895820.896.18
106Electron transfer flavoprotein beta subunit (B0SF30)9113627.325.34
107Lipoprotein LipL45 (B0SGV4)6111642.925.79
Electron transfer flavoprotein beta subunit (B0SF30)802927.325.34
108Electron transfer flavoprotein beta subunit (B0SF30)909827.325.34
109Peroxiredoxin (B0SID1)856724.575.97
110Uncharacterized protein (B0SG33)734927.057.82
111OmpL1 (B0SDB5)739231.649.02
112Hypothetical lipoprotein (B0SGX1)809733.998.83
113Endoflagellar filament core protein, FlaB1 (B0SHI7)8311231.387.03
114Flagellar filament 35-kDa core protein, FlaB2 (B0SSZ5)866431.236.55
115Endoflagellar filament core protein, FlaB-2 (B0SB70)686931.399.22
116Uncharacterized protein (B0STJ8) (Paris)c7413435.827.71
117Uncharacterized protein (B0SHJ5)685431.528.26
118*Uncharacterized protein (B0SHJ5)675531.528.26
119*DNA-directed RNA polymerase subunit alpha, RpoA (B0SA20)665436.604.86
120Elongation factor Tu (B0SAF6)503543.935.73
121Elongation factor Tu (B0SAF6)8314643.935.73
122Periplasmic protein of an ABC transporter complex (B0SE25)748543.096.35
123Bifunctional dihydrolipoyllysine-residue acetyltransferase/dihydrolipoyllysine-residue succinyltransferase (B0SEK8)565043.736.29
124Conserved hypothetical lipoprotein (B0SB57)634044.628.52
Endoflagellar motor protein (B0S9G1)615641.946.67
GDP-mannose 4,6-dehydratase (B0S9S0)664338.636.42
125*Conserved hypothetical lipoprotein (B0SB57)735744.628.52
Heavy metal efflux pump (B0SHU0)635742.287.11
NAD(P)+ transhydrogenase (AB-specific) alpha subunit (B0SH36)714040.456.78
126Uncharacterized protein (B0SC05)696843.008.79
127ATP synthase subunit beta (B0SDA5)8914251.125.28
128Acetyl-CoA acetyltransferase (B0SCQ4)757547.315.66
129LipL71 lipoprotein (B0SCA6)697551.115.42
13060-kDa chaperonin GroEL (B0SCC0)587658.115.35
131Hypothetical lipoprotein (B0S8T9)666957.396.29
132ATP synthase subunit alpha (B0SDA3)645655.086.05
133Chaperone protein DnaK (B0SHT1)619169.055.04
134Fe-S-cluster-containing hydrogenase (B0SGB2)4278113.438.48
135*Lipoprotein LipL45 (B0SGV4)273942.925.79
136*Conserved hypothetical lipoprotein (B0SCF9)656122.437.79
OmpA-family lipoprotein (B0SGK2)661320.616.62
137Hypothetical lipoprotein (B0SGB7)753719.328.98
138*50S ribosomal protein L9 (B0SB37)895616.159.75
139*Type II secretory pathway component protein G (B0S8Q5)654517.295.39
140Acetyl-CoA acetyltransferase (B0SC48)593646.066.69
141Acetyl-CoA carboxylase carboxyltransferase component subunits alpha and beta (B0SHH5)698959.926.14
142Actin-like ATPase involved in cell morphogenesis (B0SBE9)728436.775.30
143*TolC-like protein (B0SAP2)654358.438.24
144*OMA87 related protein (B0SC13)804556.879.07
145*Hypothetical lipoprotein (B0SCN0)824913.919.05
146*Uncharacterized protein (B0SGS8)891814.166.34
147*Succinate dehydrogenase/fumarate reductase subunit B (B0S8S3)913026.896.31
  • a Proteins were identified by LC-MS/MS runs on two or three independently isolated samples except for those marked with an asterisk, which indicates only a single LC-MS/MS identification. Numbers provided are from a single representative run.

  • b Calculated.

  • c Protein with the highest match was identified in the L. biflexa Paris strain.