TABLE 4

Identification of membrane-associated proteins of L. biflexa with different isoforms as determined by DIGE analysis (Fig. 3B)

SpotDescription (Uniprot accession no.)% coverageNo. of unique peptidesMass (kDa)cpIcCorresponding spot in Fig. 1a
1aChaperone protein DnaK (B0SHT1)757469.055.20MP 133
1bChaperone protein DnaK (B0SHT1)768369.055.20MP 133
2Glutamine-fructose-6-phosphate aminotransferase (B0SBL5)15867.235.75
3Glutamine-fructose-6-phosphate aminotransferase (B0SBL5)381667.235.75
4Glutamine-fructose-6-phosphate aminotransferase (B0SBL5)231067.235.75
5a60-kDa chaperonin GroEL (B0SCC0)837258.115.35MP 130
5b60-kDa chaperonin GroEL (B0SCC0)796658.115.35MP 130
5c60-kDa chaperonin GroEL (B0SCC0)817858.115.35MP 130
6aLipL71 lipoprotein (B0SCA6)675051.115.42MP 129
6bLipL71 lipoprotein (B0SCA6)676851.115.42MP 129
7aHypothetical lipoprotein (B0S8T9)806657.396.29MP 116
7bHypothetical lipoprotein (B0S8T9)816257.396.29MP 116
7cHypothetical lipoprotein (B0S8T9)8910557.396.29MP 116
8aATP synthase subunit alpha (B0SDA3)664055.086.05MP 132
8bATP synthase subunit alpha (B0SDA3)695155.086.05MP 132
8cATP synthase subunit alpha (B0SDA3)766855.086.05MP 132
10aATP synthase subunit beta (B0SDA5)663451.125.28MP 127
10bATP synthase subunit beta (B0SDA5)895451.125.28MP 127
10cATP synthase subunit beta (B0SDA5)947451.125.28MP 127
11aAcetyl-CoA acetyltransferase (B0SCQ4)835747.315.66MP 128
11bAcetyl-CoA acetyltransferase (B0SCQ4)837747.315.66MP 128
11cAcetyl-CoA acetyltransferase (B0SCQ4)909547.315.66MP 128
12aConserved hypothetical lipoprotein (B0SI06)714149.768.75
12bConserved hypothetical lipoprotein (B0SI06)663849.768.75
12cConserved hypothetical lipoprotein (B0SI06)674549.768.75
12dConserved hypothetical lipoprotein (B0SI06)805149.768.75
14aElongation factor Tu (B0SAF6)877443.935.73MP 120
14bElongation factor Tu (B0SAF6)909743.935.73MP 120
14cElongation factor Tu (B0SAF6)8813443.935.73MP 120
15aAcyl-CoA dehydrogenase (B0SF91)451442.566.43SP 35
15bAcyl-CoA dehydrogenase (B0SF91)693542.566.43SP 35
16aUncharacterized protein (B0SC05)653743.008.79MP 126
16bUncharacterized protein (B0SC05)774543.008.79MP 126
16YUncharacterized protein (B0SC05)765243.008.79MP 126
18aPeriplasmic protein of an ABC transporter complex (B0SE25)772543.096.35MP 122
18bPeriplasmic protein of an ABC transporter complex (B0SE25)874043.096.35MP 122
18cPeriplasmic protein of an ABC transporter complex (B0SE25)895943.096.35MP 122
18dPeriplasmic protein of an ABC transporter complex (B0SE25)887943.096.35MP 122
19aAldolase (B0SGS2)481438.457.71SP 30
UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase (B0SDI2)572037.066.76
N-Acetyl-gamma-glutamyl-phosphate reductase (B0SGX7)521538.126.71
19bAldolase (B0SGS2)813738.457.71SP 30
ATP-binding protein of an ABC transporter complex (B0SE24)792937.987.81
19cAldolase (B0SGS2)873838.457.71SP 30
ATP-binding protein of an ABC transporter complex (B0SE24)652037.987.81
20aUncharacterized protein (B0STJ8)–Parisb683435.827.71MP 116
20bUncharacterized protein (B0STJ8), Parisb694135.827.71MP 116
Citrate lyase beta subunit (B0SCW2)672637.106.10SP 27
20cUncharacterized protein (B0STJ8), Parisb767235.827.71MP 116
21aUncharacterized protein (B0SHJ5)893931.528.26MP 117
21bUncharacterized protein (B0SHJ5)916731.528.26MP 117
22aEndoflagellar filament core protein, flaB1 (B0SHI7)745031.387.03MP 113
22bEndoflagellar filament core protein, FlaB1 (B0SHI7)9211431.387.03MP 113
24aOmpA-family lipoprotein (B0SGK2)762820.616.62MP 103
24bOmpA-family lipoprotein (B0SGK2)8810020.616.62MP 103
26a50S ribosomal protein L7/L12 (B0SAG2)931212.655.20MP 100
26b50S ribosomal protein L7/L12 (B0SAG2)942312.655.20MP 100
27aHypothetical lipoprotein (B0SCM9)562716.657.67
Cyclic nucleotide-binding protein (B0SC72)651116.195.71
27bUncharacterized protein (B0S8Z7)54812.685.70
Hypothetical lipoprotein (B0SCM9)50616.657.67
Lipoprotein LipL45 (B0SGV4)354442.925.79MP 107
28aHypothetical lipoprotein (B0SGB7)631919.328.98MP 137
28bHypothetical lipoprotein (B0SGB7)794319.328.98MP 137
  • a The protein was also identified in the soluble fraction (SP) (Fig. 1A and Table 2) or membrane-associated fraction (MP) (Fig. 1B and Table 3).

  • b Identified only in the L. biflexa Paris strain; no hit was obtained against the Ames strain.

  • c Calculated.