TABLE 1

Primers used in this study

NameSequenceAdditional information
H1_fwAATGGTCCCAAAATTGTCColony PCR for segregation at slr0168 insertion and sequencing
H2_rvCTGTGGGTAGTAAACTGGCColony PCR for segregation at slr0168 insertion and sequencing
KanF_back_SeqTCCCGTTGAATATGGCTCColony PCR for insertion at slr0168 and sequencing
1556_Nde_FAATCATATGAAAATCGCTTTTTTTAGCAGslr1556 ORF amplification
1556_Bam_RAATGGATCCTTAATGGGGACAGATTACTTGGTslr1556 ORF amplification
1556_out_fwAATTTGGGGTGAAGCTGGGTColony PCR for slr1556 deletion
1556_out_rvCCGTTAATACTATCGTTGCTTCATTTColony PCR for slr1556 deletion
slr1556_800bp_fwAAGGTTGACCAAGGAATGGTTGGTTslr1556 knockout plasmid
slr1556_800bp_rvGTTTACGAATGGACTGATTCCACCAslr1556 knockout plasmid
slr1556_EcorI_fwGAATTCATGAAAATCGCTTTTTTTAGCSlr1556 overexpression for purification
slr1556_XhoI_revCTCGAGTTAATGGGGACAGATTACTTGGSlr1556 overexpression for purification