TABLE 3

Characterization of the kinetic properties of purified, recombinant Slr1556a

CompoundMeasured directionalityKH (mM)Vmax (μmol/mg/min)Hill coefficient
PyruvatePyruvate reductase0.39 ± 0.17236.12 ± 13.121.97 ± 0.47
HydroxypyruvateHydroxypyruvate reductase6.7 ± 1.478.14 ± 8.421.38 ± 0.24
GlyoxylateGlyoxylate reductase2.43 ± 0.01175.52 ± 12.082.34 ± 0.02
d-Lactic acidLactate dehydrogenase7.93 ± 0.2210.65 ± 0.822.52 ± 0.29
  • a The kinetic parameters for the Hill equation were calculated in SigmaPlot 11.0 as described by Goutelle et al. (31), assuming a baseline response. Data are mean values of results (n = 2).