TABLE 3

Colanic acid biosynthesis gene expression is reduced in the adrA mutant

GeneFold changeaPredicted functionb
STM14_25998.9Hypothetical protein
STM14_26145.2Hypothetical protein
gmd7.0GDP-d-mannose dehydratase
manC9.0Mannose-1-phosphate guanylyltransferase
rcsA12.4Capsular biosynthesis activation protein A
wcaA6.5Glycosyltransferase family protein
wcaB5.4 (6.1)Putative colanic acid biosynthesis acetyltransferase
wcaC5.8Glycosyl transferase family protein
wcaD7.2 (6.9)Putative colanic acid biosynthesis protein
wcaE9.5Glycosyltransferase family protein
wcaF11.5Putative colanic acid biosynthesis acetyltransferase
wcaG8.9GDP-fucose synthetase
wcaH5.4GDP-mannose mannosyl hydrolase
wcaI7.5Glycosyltransferase family protein
wcaJ3.6UDP-glucose lipid carrier transferase
wcaK5.4Putative pyruvyltransferase
wcaL4.0Putative glycosyltransferase
wcaM3.2Putative colanic acid biosynthesis protein
wza8.6Putative outer membrane polysaccharide export protein
wzb5.9Tyrosine phosphatase
wzc7.3 (10.7)Tyrosine kinase
wzxC5.9Colanic acid exporter
  • a Numbers denote reduced transcript levels in the adrA mutant as measured by microarray analysis. Numbers in parentheses denote reduced transcript levels in the adrA mutant as measured by real-time quantitative PCR.

  • b Predicted protein function information was obtained from the S. enterica serovar Typhimurium strain 14028s database on the BioCyc Database Collection website.