TABLE 2

Functional annotations of hypothetical Hpo proteins

ProteinSize (amino acids)Putative functionSuperfamily designation
Region (positions)Superfamily (specific hit/conserved domain)CDD accession no.E value
HpoR1222DNA binding response regulator17–131REC (signal receiver domain)cd001561.92E−22
161–217LuxR_C_likecd061705.87E−17
HpoR2632Signal transduction histidine kinase18–242PAS domaincd001302.26E−06
263–324HisKA (histidine kinase A)cd000821.86E−15
398–483HATPase_c (histidine kinase-like ATPases)cd000753.18E−20
515–629REC (signal receiver domain)cd001567.62E−24
HpoA494Permease8–357SLC5-6_like_sbd (nucleobase-cation symport 1 transporters)cd103232.04E−06
HpoB114Ferredoxin3–101Rieske (Rieske_RO_ferredoxin)cd035288.77E−38
HpoC409Large subunit of aromatic ring-hydroxylating dioxygenase57–183Rieske (Rieske_RO_alpha_N)cd034695.03E−33
208–404SRPBCC (RHO_alpha_C)cd006809.14E−36
HpoD116Small subunit of aromatic ring-hydroxylating dioxygenaseNo putative conserved domains have been detected
HpoE253Short-chain dehydrogenase/reductase7–248NADB Rossmann (SDR_C)cd052331.29E−61
HpoF310Ferredoxin-NADPH reductase9–213FNR_like (ferredoxin reductase/PDR_like phthalate dioxygenase reductase)cd061852.75E−86
226–304Fer2 (2Fe-2S iron-sulfur cluster binding domain)cd002073.17E−11
HpoG98Hypothetical protein2–95Dabb (stress-responsive A/B barrel domain)pfam078763.04E−19
HpoH320Cyclase52–252Cyclasepfam041991.76E−37
HpoI275Nitrilase7–269Nitrilasecd075833.2E−108