TABLE 1

Bacterial strains and plasmids used in the present study

Strain or plasmidDescriptionaReference or source
Bacterial strains
    Escherichia coli DH5αStrain used for gene cloningTaKaRa Bio Inc.
    Capnocytophaga ochracea strains
        ATCC 27872Wild typeATCC
        ΔgldK mutantermF-ermAM insertion mutation in Coch_0809 of ATCC 27872; EmrThis study
        ΔsprT mutantermF-ermAM insertion mutation in Coch_1748 of ATCC 27872; EmrThis study
        ΔsprB mutantermF-ermAM insertion mutation in Coch_0203 of ATCC 27872; EmrThis study
Plasmids
    pVA2198ermF-ermAM27
    pCR2.1-TOPO3.9-kb vector for cloning PCR products; Kmr AmprInvitrogen
  • a Antibiotic resistance phenotypes: Emr, erythromycin resistant; Kmr, kanamycin resistant; Ampr, ampicillin resistant.