TABLE 2.

Characteristics of the P. anserina hemicellulase-encoding genes selected

P. anserina geneEnzyme familyCBM familyGenBank accession no. (locus)Putative activity% Identity to T. reesei characterized GH (amino acid sequence)Condition of inductiona
PaMan5AGH5HM357135 (Pa_6_490)Endo-β-1,4-mannanase62% with TrMan5AAFMB/PX
PaMan26AGH26CBM35 (N-term)HM357136 (Pa_5_1950)Endo-β-1,4-mannanaseNAbAFMB/PX
PaXyn11AGH11CBM1 (C-term)HM357137 (Pa_2_13730)Endo-β-1,4-xylanase47% with TrXyn1, 64% with TrXyn2AFMB/PX
PaAbf51AGH51HM357138 (Pa_5_11670)α-l-ArabinofuranosidaseNAAFMB/PX
PaAbf62AGH62HM357139 (Pa_0_1370)α-l-Arabinofuranosidase35% with TrAbf2AFMB
  • a mRNA expression was induced using AFMB or pectin and xylan (PX) (see Materials and Methods).

  • b NA, not applicable. That is, no member of this family was present in T. reesei based on genomic data.